<b>Edinburgh 2014 iGEM Team: RewirED</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
<a style="width: 33%;align: left;margin-right:2%"><img src="https://static.igem.org/mediawiki/2014/a/ac/UCL2014_collaboration-start.png" style="width: 40%;"></a>
<a style="width: 33%;align: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/d/db/UCL2014_collaboration-yeey.png" style="width: 40%;"></a>
<div><h4 style="float:left;"><How it all began:</h4> <h4 style="float:right;">Making sense of antisense together!</h4></div>
<br>
<p1>The <a href="https://2014.igem.org/Team:Edinburgh">RewirED Edinburgh Team</a> focused on the creation of a metabolic wiring system as a novel way of connecting logic gates in different bacterial strains. They developed <a href="https://2014.igem.org/Team:Edinburgh/modelling/software">a software tool to analyze sequences of antisense RNA for gene silencing</a> which identifies the optimal sequence (~100bp, covering RBS and start codon) and analyses the structure to find the most stable antisense RNA. <br>
In this collaboration they provided the sequence of the antisense gene which, according to their model, has the fewest secondary structures in the core regions and analysed the behaviour of our design of an antisense gene.
<br><br>
From our side we provided real world data on the behaviour of the antisense gene silencing in order to test the accuracy of their model and efficacy of their software. Specifically we analysed the <a href="https://2014.igem.org/Team:UCL/Science/Results/Xeno#Xeno">growth</a><!--link to results--> in different media of <i>E. coli</i> engineered with the <a href="http://parts.igem.org/Part:BBa_K1336006"> antisense gene silencing BioBrick </a> <!--link to part-->. The silenced gene is core for the survival of E. coli and the reduction in growth corresponds to the efficacy of the antisense. We sent them all the data we gathered that they could then compare to their <i>in silico</i> prediction.<br>
The sequence we designed didn't effectively repress growth in <i>E.coli</i> as modelled by the software. We designed new primers <!--New primer design link!--> to amplify the sequence suggested by Edinburgh: a smaller fragment with better predicted functionality, and we are now in the stage of cloning and testing it to provide them with more data on their software's effectiveness.
<br>
</p1>
<br><br>
Identification of the optimal antisense RNA for ispB silencing
<img src="https://static.igem.org/mediawiki/2014/d/d0/UCL2014_ideal_asispB.png" width="90%" height="530">
<br><br>
Analysis of our antisense RNA design for ispB silencing
<img src="https://static.igem.org/mediawiki/2014/9/99/UCL2014_ourispBv1_structure.png" width="90%" height="550">
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-------- This is the beginning of the expanding box-------->
<div class="collapse-card"><div class="title">
<i class="fa fa-plane fa-2x fa-fw"></i><strong>
<!--- Title start --->
<b>Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
<p1>The <a href="http://met.itqb.unl.pt/">Microbial & Enzyme Technology Lab</a> led by Dr Lígia O. Martins at the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, is one of the world leaders in bioremediation with microorganisms and enzymes. Their paper titled 'Synergistic action of azoreductase and laccase leads to maximal decolourization and detoxification of model dye-containing wastewaters' <a href="http://www.ncbi.nlm.nih.gov/pubmed/21890348">[1]</a> was the fundamental inspiration for our Goodbye AzoDye project. We are truly grateful for their initial support and guidance, and for sending us the following plasmids for our experiments:<br><br>
<b>pAzoR (pLP-1)</b> containing the FMN-dependent NADH-azoreductase 1 gene. <a href="http://www.ncbi.nlm.nih.gov/pubmed/21655981">[2]</a><br>
<b>pCotA (pLOM10)</b> containing the Spore Coat Protein Laccase gene. <a href="http://www.ncbi.nlm.nih.gov/pubmed/11884407">[3]</a><br>
<b>p1B6</b> containing the mutant FMN-dependent NADH-azoreductase 1 gene <a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=Improving+kinetic+PpAzoR">[4]</a><br>
<b>pBsDyp (pRC-2)</b> containing the Dye Decolourising Peroxidase BSU38260 gene. <a href="http://www.ncbi.nlm.nih.gov/pubmed/23820555">[5]</a><br>
<b>pPpDyp (pRC-1)</b> containing the Dye Decolourising Peroxidase PP_3248 gene.<a href="http://www.ncbi.nlm.nih.gov/pubmed/23820555">[5]</a><br>
</p1><br>
<img src="https://static.igem.org/mediawiki/2014/3/3d/10491123_10204437153717396_7991059471759774917_n.jpg" width="360" height="288">
<img src="https://static.igem.org/mediawiki/2014/5/53/Group.jpg" width="360" height="288">
<img src="https://static.igem.org/mediawiki/2014/e/ed/Jointlogo.jpg" width="400" height="320">
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-------- This is the beginning of the expanding box-------->
<div class="collapse-card"><div class="title">
<i class="fa fa-camera-retro fa-2x fa-fw"></i><strong>
<!--- Title start --->
<b>Central St Martins</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
<p>We approached the Central St Martins textiles department with our ideas of synthetic biology and science and they asked ‘When does technology like this become accessible?’ This question yielded a set of beautiful visualisation of the way our bacteria could be used to create art if controlled by light. These pieces by second year Textiles Design BA students Cameo Bondy and Barbara Czepiel exhibit the textiles that could be created if our bacteria contained optogenetic biobricks that switched their dye breakdown capacities on and off via light cues. </p>
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-------- This is the beginning of the expanding box-------->
<div class="collapse-card"><div class="title">
<i class="fa fa-picture-o fa-2x fa-fw"></i><strong>
<!--- Title start --->
<b>Natsai Audrey</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
<p>A practicing independent designer and researcher, Natsai Audrey Chieza is a Design Futurist inspired by material innovation and technology. Natsai considers her creative pursuits with a strong interest in how the life sciences can enable new craft processes for a more robust environmental paradigm.</p>
<br>
<p>Natsai contributed a series of pieces to be displayed at the #UncolourMeCurious from her Faber Futures exhibition, exploring the use of bacteria to create pigments and dye fabrics, deviating from the standardisation of a petri dish.</p>
<br>
<p>Natsai has achieved measurable success in design research projects for Microsoft, Nissan, Unilever and EDF Energy. She has also exhibited in numerous design exhibitions and events across Europe including the Victoria & Albert Muesum, London; Audax Textile Museum, Tilburg; Salone Internazionale del Mobile di Milano, Milan; Designersblock LDF, London; EN VIE/ ALIVE, Paris; Science Gallery, Dublin; and Heimtextil, Frankfurt.</p><br>
<div class="inlinegb">
<img src="https://static.igem.org/mediawiki/2014/a/af/Natsai_Final.png" width="100%">
</div>
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-------- This is the beginning of the expanding box-------->
<div class="collapse-card"><div class="title">
<i class="fa fa-university fa-2x fa-fw"></i><strong>
<!--- Title start --->
<b>University of Westminster</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
<table style="width:100%"><col width="30%"><col width="70%">
<tr><td>
<!---Sponsor Image Start--->
<img src="https://static.igem.org/mediawiki/2014/thumb/4/4c/10509555_10152632952558343_8803867533531068741_n.png/337px-10509555_10152632952558343_8803867533531068741_n.png" style="margin:0 0 0 15px;" width="80%" alt="UCL Biochemical Engineering" style="max-width: 100%;"></a>
<!---Sponsor Image End--->
</td><td>
This year the UCL iGEM Bioprocess Team paid a visit to Godfrey Kyazze, a Lecturer in Bioprocess Technology at University of Westminster. He is involved in water science research, using microbial fuel cells to produce electricity upon the degradation of azo dyes by bacterial cells. We greatly appreciated the opportunity to speak to him about his research, go into the lab and see some real examples of fuel cell modules. Through the visit, the team has certainly gained a valuable perspective on the potential application of azo dye degradation, not only for environmental remediation, but also for the production of energy.
<!---Sponsor Description End--->
</td>
</tr></table>
<!---SPONSOR END--->
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-------- This is the beginning of the expanding box-------->
<div class="collapse-card"><div class="title">
<i class="fa fa-picture-o fa-2x fa-fw"></i><strong>
<!--- Title start --->
<b>Linden Gledhill</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
*EDO CONTENT*
INSERT VIDEO HERE EDO!!!
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-------- This is the beginning of the expanding box-------->
<div class="collapse-card"><div class="title">
<i class="fa fa-graduation-cap fa-2x fa-fw"></i><strong>
<!--- Title start --->
<b>The Slade</b>
<!--- Title end --->
</strong></div><div class="body">
<!--- Content start--->
<p>For the exhibition The Slade School of Art provided us with Pigment Cases outlining the history of dyes. They illustrated how dyeing technology has moved through the ages and allowed the public to witness how far we have come. </p>
<!--- Content end--->
</div></div><br>
<!--------- This is the end of the expanding box-------->
<!-- =========================STOP========================== -->
</div><!-- This is the css of the page. Dont change it unless you have consulted with Lewis or Adam about what your changing-->
</div>
<a href="#" class="back-to-top"><img src="https://static.igem.org/mediawiki/2014/5/5d/Scroll_Up_Button.png" width="64" height="64"></a>
<script type="text/javascript">
jQuery(document).ready(function() {
var offset = 220;
var duration = 500;
jQuery(window).scroll(function() {
if (jQuery(this).scrollTop() > offset) {
jQuery('.back-to-top').fadeIn(duration);
} else {
jQuery('.back-to-top').fadeOut(duration);
}
});
jQuery('.back-to-top').click(function(event) {
event.preventDefault();
jQuery('html, body').animate({scrollTop: 0}, duration);
return false;
})
});
</script>
<style>
/*=======PAGE HEADER=======*/
.pageTitle {
height:200px;
width:100%;
background-color:darkgrey;
padding-top:50px;
display:inline-block;
}
.floater {
float:left;
}
/*=======Body=======*/
.textArena {
background-color:white;
padding: 5% 5% 5% 5%;
font-size:90%;
font-family: 'Open Sans', 'Helvetica Neue', Helvetica, Arial, sans-serif;
}
.widthCorrect {
width:100%;
}
/*=========Top Gap div id from Oran=============*/
#TopGapO {
height: 70px;
width: 100%;
background: black;
}
.back-to-top {
position: fixed;
bottom: 0em;
right: 0.5em;
background-color: #AA8799;
text-decoration: none;
color: #000000;
display: none;
border-radius: 100px;
padding-bottom: 25px;
margin-bottom: -25px;
z-index: 99999;
}
</style>
<footer style="margin-bottom: -31px;">
<div class="wrapper clearfix">
<div class="FooterLogos">
<div id="FL1"><a href="http://www.facebook.com/UCLiGEM"><img src="https://static.igem.org/mediawiki/2014/5/50/Facebook_iconTran.png" height="30px" width="30px" style="padding-top: 15px;"></img></a></div>
<div id="FL2"><a href="http://www.goodbyeazodye.org.uk/"><img src="https://static.igem.org/mediawiki/2014/9/9a/TumblrTran.png" height="60px" width="60px"></img></a></div>
<div id="FL3"><a href="http://twitter.com/UCLiGEM"><img src="https://static.igem.org/mediawiki/2014/a/a6/Twitter_iconTran.png" height="30px" width="30px" style="padding-top: 15px;"></img></a></div>
</div>
<div class="FooterContent">
<div id="FC11" style="font-size:120%">
<h3 class="fontfixer" style="margin-top: 5px;">Contact Us</h3>
<p class="fontfixer">University College London <br> Gower Street - London <br> WC1E 6BT<br>Biochemical Engineering Department<br>
<strong>Phone:</strong> <a href="tel:phonenum" class="tele">+44 (0)20 7679 2000</a><br>
<strong>Email:</strong> <a href="mailto:email@domain.com">ucligem2014@gmail.com</a></p>
</div>
<div id="FC22">
<h3 class="fontfixer" style="margin-top: 5px;"><a href="https://2014.igem.org/Team:UCL/Humans/Sponsor" style="color: black;">Sponsors</a></h3>
<table width="100%">
<tr>
<td><a href="http://www.bbsrc.ac.uk/home/home.aspx"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/7/74/BBSRC_logo_2.png" alt="" width="100%" style="
max-width: 184px;"></a></td>
<td><a href="http://www.biochemistry.org/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/d/d7/BioChem_Soc_logo_2.png" alt="" width="100%" style="
max-width: 184px;"></a></td>
<td><a href="http://www.sgm.ac.uk/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/e/ee/MicroBio_logo_2.png" alt=" " width="100%" style="
max-width: 184px;"></a></td>
</tr>
<tr>
<td><a href="http://www.wellcome.ac.uk/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/4/43/Wellcome_Trust_logo_2.png" alt=" " width="100%" style="
max-width: 184px;"></a></td>
<td><a href="http://www.engineering.ucl.ac.uk/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/1/1a/UCL_Eng_logo_2.png" alt=" " width="100%" style="
max-width: 184px;"></a></td>
<td><a href="http://www.neb.com/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/0/06/NEB_logo.png" alt=" " width="100%" style="
max-width: 184px;"></a></td>
</tr>
<tr>
<td><a href="http://www.synbiota.com/welcome_page/welcome"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/8/88/Synbiota.png" alt="" width="100%" style="
max-width: 184px;"></a></td>
<td><a href="http://www.snapgene.com/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/5/50/SnapGene_Logo_288.png" alt="" width="100%" style="
max-width: 184px;"></a></td>
<td><a href="http://www.geneoracle.com/"><img class="alignleft" src="https://static.igem.org/mediawiki/2014/7/78/GeneOracle_logo.png" alt="" width="100%" style="
max-width: 184px;"></a></td>
</tr>
<!--<tr>
<td><a href=""><img class="alignleft" src="" alt="" width="100%"></a></td>
</tr>-->
</table>
</div>
<!--YK>LM: Why is this div 446 x 250, and div id="FC11" and id="FC22" at 446 x 300? LM: the twitter thing has a set height which buggers up the rest of the standardized height-->
<div id="FC33">
<h3 class="fontfixer" style="margin-top: 5px;">Follow Us</h3>
<a class="twitter-timeline" href="https://twitter.com/UCLiGEM" data-widget-id="494172163977392128" height="235px" width="300px">Tweets by @UCLiGEM</a>
<script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script>
</div>
</div>
</div>
</footer>
<style>
.fontfixer {
font-family: 'Open Sans', 'Helvetica Neue', Helvetica, Arial, sans-serif;
}
</style>
</p>
<!--
NewPP limit report
Preprocessor node count: 19/1000000
Post-expand include size: 120/2097152 bytes
Template argument size: 0/2097152 bytes
Expensive parser function count: 0/100
-->
<div class="printfooter">
Retrieved from "<a href="http://2014.igem.org/Team:UCL/Humans/Collab">http://2014.igem.org/Team:UCL/Humans/Collab</a>"</div>
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
<div class="visualClear"></div>
</div>
</div>
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR -->
<div class="visualClear"></div>
<div id='footer-box' class='noprint'>
<div id="footer">
<div id="f-poweredbyico"><a href="https://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div> <div id="f-copyrightico"><a href="https://creativecommons.org/licenses/by/3.0/"><img src="https://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div> <ul id="f-list">
<!-- Recentchanges is not handles well DEBUG -->
<li id="t-recentchanges"><a href="/Special:RecentChanges"
title='Recent changes'>Recent changes</a></li>
<li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:UCL/Humans/Collab"
title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
<li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:UCL/Humans/Collab"
title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
<li id="t-specialpages"><a href="/Special:SpecialPages"
title="List of all special pages [q]" accesskey="q">Special pages</a>
</li>
<li><a href='/Special:Preferences'>My preferences</a></li>
</ul>
</div> <!-- close footer -->
<div id='footer'>
<ul id="f-list">
<li id="t-print"><a href="/wiki/index.php?title=Team:UCL/Humans/Collab&direction=prev&oldid=370375&printable=yes"
title="Printable version of this page [p]" accesskey="p">Printable version</a>
</li>
<li id="t-permalink"><a href="/wiki/index.php?title=Team:UCL/Humans/Collab&oldid=370268"
title="Permanent link to this revision of the page">Permanent link</a>
</li>
<li id="privacy"><a href="/2014.igem.org:Privacy_policy" title="2014.igem.org:Privacy policy">Privacy policy</a></li>
<li id="disclaimer"><a href="/2014.igem.org:General_disclaimer" title="2014.igem.org:General disclaimer">Disclaimers</a></li>
</ul>
</div> <!-- close footer -->
</div> <!-- close footer-box -->
<script>if (window.runOnloadHook) runOnloadHook();</script>
</div>
<!-- Served in 0.307 secs. --></body>
</html>
"
