From 2014.igem.org

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20^th June

• Preparation of samples :

1st sample :
• Pellet of 2ml
• Add SB1X
• Add beads => vortex
• 5 min at 85°C
2nd sample 
• 2ml of purification product
• 10µL of sample
• 10µl of SB 2X
• Exposure at CuCl2
=> SDS PAGE Digestion psB6A1 (miniprep 10/06/14) and VPGXG 20 (PCR 10/06/14)

	Plasmid digestion	Insert digestion
H2O	5,6µl	16,38µl
10X buffer 4	2µl	2µl
XbaI	0,25µl	0,25µL
EcoRI	0,25µl	0,25µl
DNA	11,9µl	1,12µl

1h at 37°C + 5µl of digestion -> electrophoresis
• ELP
  • To quantify => scale ELP pellets
  • HPLC
  • Find a solvant who precipitate ELP immediately.
  Electrophoresis of PCR of 19/06/14 and VPGXG 20 of 16/06/14