Team:Bordeaux/NotebookJune20
From 2014.igem.org
20th June
Molecular biology
- Preparation of samples :
- Pellet of 2ml
- Add SB1X
- Add beads => vortex
- 5 min at 85°C
- 2ml of purification product
- 10µL of sample
- 10µl of SB 2X
- Exposure at CuCl2
- ELP
- To quantify => scale ELP pellets
- HPLC
- Find a solvant who precipitate ELP immediately.
Electrophoresis of PCR of 19/06/14 and VPGXG 20 of 16/06/14
1st sample :
Digestion psB6A1 (miniprep 10/06/14) and VPGXG 20 (PCR 10/06/14)
Plasmid digestion | Insert digestion | |
---|---|---|
H2O | 5,6µl | 16,38µl |
10X buffer 4 | 2µl | 2µl |
XbaI | 0,25µl | 0,25µL |
EcoRI | 0,25µl | 0,25µl |
DNA | 11,9µl | 1,12µl |