Team:Groningen/Template/MODULE/Notebook/bandage/week14

This week new cultures were grown for freeze drying. This was done to test the growth in chemically defined medium (CDM), which is the medium that will be used in the final bandage. Cultures with several OD₆₀₀ values were freeze dried in 5 ml samples. Unfortunately the freeze drying machine could only be used on the 9^th of October. Samples were taken from the machine on the 10^th of October and one of the samples was resuspended in 1 ml demi water to check for viability. The resuspended cells were inoculated in CDM in a 1:100 ratio and grown overnight at 200 rpm and 30 C.

Growth was visible in the medium the next morning. This means the cells were viable and therefore the freeze drying of Lactococcus lactis was successful.