Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs

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Strains


General

Module 1

Module 2

Module 3

Biosafety

Name Properties / Genotype BioBrick / Reference
General
Escherichia coli KRX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase Promega
E. coli DH5α F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 invitrogen
Module 1
E.coli KRX ΔdcuB::oprF [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdcuB::oprF BBa_K1172507
JW4084-1 ΔdcuB749::kan Baba et al., 2006
JW0506-1 ΔfdrA730::kan Baba et al., 2006
Actinobacillus succinogenes 130Z wild type Guettler et al., 1999
Geobacter sulfurreducens PCA wild type Viulu et al., 2013
Module 2
E.coli BL21 F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) NEB
Module 3
JW1228-1 ΔadhE748::kan Baba et al., 2006
Biosafety
E. coli KRX Δalr [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, Δalr
E. coli KRX ΔdadX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX
E. coli KRX Δalr ΔdadX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX, Δalr
E. coli DH5α Δalr F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr
E. coli DH5α ΔdadX F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 ΔdadX
E. coli DH5α Δalr ΔdadX F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr ΔdadX




Constructs


General

Module 1

Module 2

Module 3

Biosafety

Name Properties BioBrick / Reference
General
_
Module 1
_
Module 2
pHnCBS1D cbbL-cbbS-csoS2-csoS3-csoS4A-csoS4B-csoS1C-csoS1A-csoS1B-csoS1D, CmR Bonacci et al., 2012
pET16b-glpXC AmpR, glpXC encodes FBPase/SBPase of Bacillus methanolicus Stolzenberger al.,2013
pSB1A2-T7:prkA AmpR, CDS of phosphoribulokinase (prkA) from Synechococcus elongatus under the T7 promoter
pSB1A2-T7:Hneap AmpR, CDS of RubisCO (Hneap) from Halothiobacillus neapolitanus under the T7 promoter
Module 3
pSB1A2_T7_alsS_ilvC_ilvD_kivD Isobutanol production pathway under the T7 promoter
pSB1A2_T7_alsS_ilvC_ilvD_kivD Isobutanol production pathway including the adhA from L. lactis under the T7 promoter
Biosafety
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References
  • Stolzenberger, J., Lindner, S.N., Persicke, M., Brautaset, T., and Wendisch, V.F. (2013). Characterization of Fructose 1,6-Bisphosphatase and Sedoheptulose 1,7-Bisphosphatase from the Facultative Ribulose Monophosphate Cycle Methylotroph Bacillus methanolicus. J Bacteriol 195, 5112–5122.
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
  • Guettler, M. V., Rumler, D., Jain, M. K. (1999). Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing bacterium from the bovine rumen. Int.J.Syst.Bacteriol. 49 : 207-216.
  • Viulu, S., Nakamura, K., Kojima, A., Yoshiyasu, Y., Saitou, S., Takamizawa, K. (2013). Geobacter sulfurreducens subsp. ethanolicus, subsp. nov., an ethanol-utilizing dissimilatory Fe(III)-reducing bacterium from a lotus field. J Gen Appl Microbiol 59 ( 5 ): 325-34.

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