We designed four reformed plasmids, pBluescript II KS(+) ScaI deletion, pBluescript II KS(+) EcoRV deletion, pBluescript II KS(+)_3_copy, pBluescript II KS(+)_5_copy.
And we test their co-transformation ability and get the result picture. All of these plasmid has Amp antibiotic resistance.
Figure 2.1.1 Four plate of Co-transfected bacteria
So we experimentally validate that our reformed plasmids perform as expected.
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