Team:HZAU-China/Design

Design Overview

Our system of the rewirable circuit is composed of three modules, including an input module, a processing module, and an output module. The input module acts as a sensor and a trigger. It receive the environment signal and expresses recombinase to rewire the processing module. The processing module is a small gene regulatory network with some specific sites for DNA recombination that lead to rearranging the connection of parts. In order to confirm the functions, we need an output module to monitor the real-time processes. Here we use fluorescence intensity as an indicator. This is the first step. Once its function is confirmed, we can use other functional proteins as output to solve real world problems.

To mimic the intricacies of nature, we kept the following concepts in mind when designing the circuits: