Team:SF Bay Area DIYbio/Notebook

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Notebook

General Protocols

• Electra Cloning Protocol
  
• DNA Handling Protocol
  
• Media:Zymo E. coli competent cells transformation protocol.pdf
  
• Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  
• Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
  
• Media:Sequencing_Analysis_using_Serial_Cloner_2_16Oct2014.pdf‎
  

General Documentation Information

• Real Vegan Cheese Notebook Documentation Guidelines
  

Experiments

June 2014

• CR_062214_humanKappaCasein_001
  

August 2014

• CLONE_082514_humKappaCasein(Kex+)
  
• Growth Curve Experiment
  

September 2014

• Cloning 7 casein gBlocks, 12 Sep 2014
  
• Troubleshooting failed transformation of P.FAKS.humBeta.S on 12 Sept. 2014
  
• Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  
• Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
  

October 2014

• Initial studies of yeast strains' growth conditions and troubleshooting Oct.2
  
• Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  
• Cloning human alpha casein S1, 04Oct2014
  
• Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 08Oct2014
  
• Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014
  
• Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 10Oct2014
  
• Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 13Oct2014
  
• Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014
  
• Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014