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From 2014.igem.org

WELCOME TO iGEM 2014! Your team has been approved and you are ready to start the iGEM season! On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! Click here to edit this page!

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Notebook

General Protocols

• Electra Cloning Protocol
  
• DNA Handling Protocol
  
• Media:Zymo E. coli competent cells transformation protocol.pdf
  
• Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  
• Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
  

General Documentation Information

• Real Vegan Cheese Notebook Documentation Guidelines
  

June 2014

• CR_062214_humanKappaCasein_001
  

August 2014

• CLONE_082514_humKappaCasein(Kex+)
  
• Growth Curve Experiment
  

September 2014

• Cloning 7 casein gBlocks, 12 Sep 2014
  
• Troubleshooting failed transformation of P.FAKS.humBeta.S on 12 Sept. 2014
  
• Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  
• Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
  

October 2014

• Initial studies of yeast strains' growth conditions and troubleshooting Oct.2
  
• Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  
• Cloning human alpha casein S1, 04Oct2014
  
• Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 08Oct2014
  
• Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014
  
• Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 10Oct2014
  
• Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 13Oct2014
  
• Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014
  
• Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014