Week Three

Monday, 6/30/14

• Discussion of fallout from Friday's meeting
• More paper-reading to determine what's wrong with our new quorum-sensing system candidates
• Designed geneblocks for our new systems and subgroup projects

Tuesday, 7/1/14

• Lab cleanup. Reorganized -20 freezer samples.
• World Cup watching. Tim Howard's a boss
• Work on the team wiki while we wait for our next batch of geneblocks to ship

Wednesday, 7/2/14

• PCR'ed out LacI and TetR genes from pAA001 plasmid due to sequence similarity problems
• Inserted new pAA001 plasmids into competent cells and plated<\li>

Thursday, 7/3/14

• Picked 12 colonies from yesterday's transformed cells and performed colony PCR

Friday, 7/4/14