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Team:Bielefeld-CeBiTec/Notebook/Journal/CO2-fixation/Oct
From 2014.igem.org
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October |
- tkt
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- fba
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- glpX
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- prkA and ptac_Hneap
- We tried to bring the prkA with RBS behind our construct ptac_Hneap but it was not successful.
- prkA
- We tried to bring our prkA with RBS in pSB1C3.
- BioBrick Assembly (Suffix)
- Transformation with chemocompetent cells
- Plasmid isolation of [Construct]
- Restriction digestion with PstI and EcoRI
- Bands as expected (~2000 bp and ~1100 bp)
