07/05/2014

Results with the antibiotics tests:

Legend

Green: it grew.

Yellow: It didn’t grow at a good amount

Red: It didn’t grow

Pictures of our trials using antibiotic in the JM110 e ERC2925 E. coli lines ER5C2925 – Trial with Streptomycin, in order: 20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml:

ER5C2925 – Trials with Ampicillin: 50ug/ml and 100ug/ml:

ER5C2925 – Trials with Tetracycline : 12 ug/ml and 24 ug/ml:

ER5C2925 – Trials with Kanamycin: 25 ug/ml and 50 ug/ml:

ER5C2925 – Trials with Chloramphenicol: 34 ug/ml and 68 ug/ml:

JM110 – Trials with Streptomycin: 20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml:

JM110 – Trials with Tetraciclyn:12 ug/ml and 24 ug/ml:

JM110 – Trial with Ampicylin 50 ug/ml 100 ug/ml:

JM110 – Trial with Kanamycin: 25 ug/ml and 50 ug/ml:

JM110 – Trials with chloramphenicol: 34 ug/ml and 68 ug/ml:

Today we also cryopreserved the bacteria with glycerol and we inoculated LB soft medium to store them.

We transformed using the Transformation Protocol using Sorbitol in the li E. coli JM110 e ERC2925 lines with the pUC72 plasmid.