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Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Sep
From 2014.igem.org
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September |
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- adhA
- This week we tried to verify the positive cutures that were identified last week by a restriction digest
- Plasmid isolation of pSB1K3_adhA
- Restriction digestion with EcoRI and PstI of pSB1K3_adhA
- Bands as expected (~1200 bp)
- Successful sequencing
Agarose gel from the restriction digestion with EcoRI and PstI. As a Ladder we used GeneRuler™ 1 kb DNA Ladder from Thermo Scientific.
- T7_alsS_ilvC_ilvD_kivD
- This week we tried to verify the positive cutures that were identified last week by a restriction digest
- Plasmid isolation of pSB1A2_T7_alsS_ilvC_ilvD_kivD
- Restriction digestion with EcoRI and PstI of pSB1A2_T7_alsS_ilvC_ilvD_kivD
- Bands not as expected (~7200bp)
