The project X-press: Characterization of pSBX-vectors made by Team Heidelberg
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.
In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.
The Team Heidelberg expression vectors
These are the tested constructs:
| Plasmid | Backbone | Insert | Length vector | Length Insert | Resistance
|
| pSBX1A3-BBa_13507 | pSBX1A3 (high copy) | BBa_13507 | 3067 bp | 1226 bp | 50 mg/ml Ampicilin
|
| pSBX1C3-BBa_13507 | pSBX1C3 (high copy) | BBa_13507 | 2982 bp | 1226 bp | 35 mg/ml Chloramphenicol
|
| pSBX1K3-BBa_13507 | pSBX1K3 (high copy) | BBa_13507 | 3116 bp | 1226 bp | 50 mg/ml Kanamycin
|
| pSBX1T3-BBa_13507 | pSBX1T3 (high copy) | BBa_13507 | 3322 bp | 1175 bp | 10 mg/ml Tetracyclin
|
| pSBX4A5-BBa_13507 | pSBX4A5 (low copy) | BBa_13507 | 4307 bp | 1192 bp | 50 mg/ml Ampicilin
|
| pSBX4C5-BBa_13507 | pSBX4C5 (low copy) | BBa_13507 | 4127 bp | 1186 bp | 15 mg/ml Chloramphenicol
|
| pSBX4K5-BBa_13507 | pSBX4K5 (low copy) | BBa_13507 | 4331 bp | 1192 bp | 50 mg/ml Kanamycin
|
| positive control: RFP selection marker with constant expressin of mRFP, 15 mg/ml Chloramphenicol
|
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