Overview
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
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Week Thirteen |
Sunday, 9/7/14
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Monday, 9/8/14
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lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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Tuesday, 9/9/14
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Export Systems
Preparation of fsr system for LC/MS analysis:
- Made new LB-CARB plates, since the old stock had run out.
- Transformed pTG005-unFLAG into DH5α. Cells were plated & incubated overnight.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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Wednesday, 9/10/14
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Export Systems
Preparation of fsr system for LC/MS analysis:
- Set up a liquid culture of pTG005-unFLAG cells.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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Thursday, 9/11/14
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Export Systems
Preparation of fsr system for LC/MS analysis:
- Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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Friday, 9/12/14
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Export Systems
Preparation of fsr system for LC/MS analysis:
- aTc-induced MOPS liquid cultures from yesterday were spun down, and then the supernatant was loaded into C18 columns, washed with 10% acetonitrile, and then eluted with 60% acetonitrile.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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Saturday, 9/13/14
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Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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