Team:CityU HK/notebook/lablog/project1

Week 3 (13/7~19/7)

- Primer design for leaderless tesA for Fit-coli (‘tesA) & New biobrick (‘tesA BB)

Week 4 (20/7~26/7)

- PCR of ‘tesA and ‘tesA BB

- PCR purification of ‘tesA and ‘tesA BB

- Prepare LB agar plates(‘tesA BB)

Week 5 (27/7~31/7)

- Digestion of ‘tesA BB and pSB1C3 plasmid with EcoRI & PstI restriction enzymes

- PCR purification for digested ‘tesA BB

- Sub-cloning of ‘tesA BB into pSB1C3 plasmid

- Transformation of ‘tesA BB into W3110 E.coli

- Pick colonies of the transformed E. coli with ‘tesA BB

O my friend - but it is too much for my strength - I sink under the weight of the splendour of these visions!

A wonderful serenity has taken possession of my entire soul, like these sweet mornings of spring which I enjoy with my whole heart.

I am alone, and feel the charm of existence in this spot, which was created for the bliss of souls like mine.

The bedding was hardly able to cover it and seemed ready to slide off any moment. His many legs, pitifully thin compared with the size of the rest of him, waved about helplessly as he looked. "What's happened to me?"

He thought. It wasn't a dream. His room, a proper human room although a little too small, lay peacefully between its four familiar walls.