Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Jul

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July

  • kivD, alsS, ilvC, ilvD and backbone of pSB1C3
    • We tried to redo the amplification of last week.
      • Optimization of PCR conditions for coding sequence amplification
        • combinations of primers and templates as described before
        • annealing temperature gradients from 50°C to 58°C were tried
        • product amount was increased by lower annealing temperatures
  • kivD, alsS, ilvC, ilvD and backbone of pSB1C3
    • We tried to redo the amplification of last week.
      • Optimization of PCR conditions for coding sequence amplification
        • combinations of primers and templates as described before
        • annealing temperature gradients from 50°C to 58°C were tried
        • product amount was increased by lower annealing temperatures
          • 54°C was identified as optimal annealing temperature
          • 90 seconds were identified as optimal elongation time