"
Team:Bielefeld-CeBiTec/Notebook/Primer
From 2014.igem.org
Primerlist
| Primer ID | Primer sequence |
|---|---|
| Module 1 | |
| Gene synthesis | |
| pSB1C3_pre_Fum_A (43 bp) | 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_Fum_A (43 bp) | 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_Fum_BCD (42 bp) | 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘ |
| pSB1C3_suf_Fum_BCD (43 bp) | 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| CCM-Cassette(E. coli) | |
| ccm_fwd (58 bp) | 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘ |
| ccm_rev (47 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘ |
| pSB1C3_suf_ccm (50 bp) | 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccm (28 bp) | 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘ |
