From 2014.igem.org
Thursday, August 14, 2014
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Today's tasks:
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- Miniprep ctxA, ctxB.
- Test digest and ran gel for ctxA, ctxB.
- Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
- Prepared for sequencing: diluted to the correct volume and concentration.
- Purified RBS (digested overnight yesterday).
- Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
- Inoculate more colonies from ctxA, ctxB plates.
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RBS-Carb Gel was good.
Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.
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