Team:Nagahama

From 2014.igem.org

One Gene Has One Function

What is us project at 2014? Cadmium is one of a harmful material for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. And why itai-itai diserse was going around? The cause is industrial wastewater. Cadmium is contained the water. This water needs to be clean. Therefore, we thought how to clean the water. We think of be use E.coli. Concretely, we use two kinds of E.coli. One of E.coli catches Cadmium. Other close to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli). To use these E.coli. Finally cadomiun is catched(wastewater is be clean).

What experiment we did?

・how to check E.coli's chemotaxis We did other igem team’s　assay. Use to soft agar plate.this plate let E.coli siwm.

・made a plasmid Our project need to two kind of plasmid. we made one plasmid.And we have to make one more plasmid.