Team:TU Eindhoven
From 2014.igem.org
TU Eindhoven iGEM 2014 Project
Our goal this year in the iGEM competition is to create a new tool for the synthetic biologist’s toolbox; a tool that allows genetically engineered machines to operate inside the human body without causing an immune response. We want to achieve this by combining polymer chemistry and microfluidics to create individually encapsulated bacteria. Want to learn more? Go to our Project Overview !
Microfluidics
We are busy optimizing our droplet device to get the right parameters for our final device. The design for this device is also being finalized. The device will be used to create droplets with a few bacteria inside in which the polymer chemistry will be done. After the reaction we will test the formation of the encapsulation with FACS.
Labwork
Currently we are making our designed plasmids, after the sequencing results we will hopefully be able to start with protein expression and turning our designed genes into biobricks!
Modeling
We are currently busy researching possible modelling projects, come back later for more information!
Human Practices
For human practices two big projects are currently in focus: creating a picture book about lab work for our younger audience, and building a game about synthetic biology for middle school students.
Team News
Microfluidics: Testing with polyacrylamide droplets is going well.
- 31 July 2014
- 25 July 2014
Lab work: FACS analysis using fluorescent DBCO molecules shows that our modified protein is expressed on the membrane, we are now testing with DBCO molecules with PEG tails.
Lab work: Click reaction results show that the product forms, optimization of the reaction is now underway.
- 29 July 2014
- 22 July 2014
Human practices: our interactive synthetic biology game is in its bèta phase!
Created by iGEM Team TU Eindhoven 2014