most of the existing genetic engineering has been done with E.Coli

but what if we could work in more organisms?

The goal of our project was to explore and compare the different transcriptional and translational rates of known model organisms such as E. coli to various non-model strains, with all processes taking place in cell free systems. This is in the hopes that by compiling a list of well-characterized parts such as promoters and RBSs, the information could be used to further the field of synthetic biology through environmental, health, and industrial applications by eliminating the need to modify E. Coli to meet particular environmental settings

The possibilities through synthetic biology are endless, but oftentimes the foundational scientific methods are not fully optimized or explored. Each project operates fairly autonomously, pulling on the work of others to aid research, yet sometimes plowing ahead to solve the problem at hand without the contributions from predecessors. The most categorized and recognized promoters are the Anderson Promoters (site/is this even a valid claim?). (there were also many papers that Joe sent which talk about parts categorization) However, the rank classification for these promoters are based on the relative strength of maximum fluorescence and nothing else.

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