The objective in April, while classes were still in session, was to make something called the Z4EV-dCas9-MXI1 construct. The model organism used was yeast, and this was a continuation of last year’s project The general idea was to fuse a repressor domain onto the dCas9 gene, to make an even stronger repressor. MXI1 is the repressor domain, and Z4EV is the promoter that was used. It’s originally a zinc-finger induced promoter for yeast. This approach was abandoned because the general consensus was that repression is harder to achieve in yeast. Instead of trying to continue last year’s project, we decided to start afresh, with a new model organism, E. coli.

This is what we were trying to accomplish in May and June. May and June Lab Notebook

This is what we were trying to accomplish in July. July Notebook

This is what we were trying to accomplish in August. August Lab Notebook

This is what we were trying to accomplish in September. September Lab Notebook

This is what we were trying to accomplish in October. October Lab Notebook

Protocols can be found here