Team:Caltech/week11

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Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week Eleven

Monday, 8/25/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Tuesday, 8/26/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Wednesday, 8/27/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Thursday, 8/28/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Re-ran a Gibson assembly of pAA009
  • Transformed Gibson products into JM109 cells

Friday, 8/29/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Picked 10 Gibson colonies from yesterday
  • Ran colony PCR on re-suspended colonies, inoculated liquid cultures of picked colonies
  • Ran gel of colony PCR products, but no good bands were obtained

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