Team:Jilin China/Outline

May, 2014

1. Data query and project theme select
2. Experiment scheme design
3. Codon optimization of MlrA gene by Lactococcus lactis codons）
4. MlrA gene sequence design and split
5. RFP-Mlr-GFP sequence design and split

June, 2014

1. MlrA gene synthesis and sequence
2. RFP-Mlr-GFP gene synthesis and sequence

July, 2014

1. MlrA gene expression and detection
2. RFP-Mlr-GFP gene expression and detection
3. Screening of microcystin LR sensitive promoter

August, 2014

1. construction of the recombinant vector pMG-mlr
2. Recombinant vector was transformed into Escherichia coli
3. GFP-mlrA expression by mlr promoter and detection in Escherichia coli
4. Detection of microcystins LR degradation ability in water by Recombinant Escherichia coli

September, 2014

1. Recombinant vector was transformed into Lactococcus lactis
2. GFP-mlrA expression by mlr promoter and detection in Lactococcus lactis
3. Detection of microcystins LR degradation ability in water by Recombinant Lactococcus lactis

October, 2014

1. Detection of microcystins LR degradation ability in the natural environment by Recombinant Escherichia coli
2. Detection of microcystins LR degradation ability in the natural environment by Recombinant Lactococcus lactis