Team:Hong Kong HKUST/riboregulator/future work
From 2014.igem.org
Riboregulator Future Work
Catalog Page
Since the number of regulatory RNAs in part registry have been increasing over the years and they have not been well curated, our team wishes to solve this problem by creating a list of category tags and guidelines so that one can search these parts efficiently. Therefore, we will be continuing our work in testing and characterizing the regulatory RNAs for the ease of the synthetic biology community's use in the future. Please visit our prototype of the catalog page and stay tuned with our updates. |
Re-characterization of Riboregulator under different genetic context
The characterization result of riboregulators were out of our expectation - the taRNAs were unable to efficiently unlock their cognate crRNAs. We now realized that our genetic context used was different from how riboregulators were characterized initially. Expression of TAs were supposed to be driven from high copy plasmids while CRs were supposed to be driven from low copy vectors. Thus, we will progressively change the conditions used to characterize the constructs into a new genetic context. We hope that we can replicate others' results soon. |
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