Data and submitted physical DNA for composite constructs used in characterization:
- Lock 1, BBa_J01008 (Golden Bear, 2005): BioBricked version of Isaacs' riboregulator trans-activating key, taR12
- Lock3c, BBa_J23031 (Team Berkeley iGEM, 2006): lock3c.
- Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP., BBa_J23008 (Berkeley iGEM, 2006): Key 3c
Parts with qualified sample in the Part Registry:
- Lock 3C RBS, BBa_K1379015: B0032 derivate for Lock 3C.
- HKUST Lock 1, BBa_K1379018: Cis-Repressor HKUST Lock 1.
- HKUST Key 1, BBa_K1379019: Trans-Activator for HKUST Key 1.
- Cis-repressed GFP Lock 1, BBa_K1379020: Cis-repressed GFP Generator Lock 1
- Cis-repressed GFP Generator Lock 3, BBa_K1379024: Cis-repressed GFP Generator Lock 3C
- Cis-repressed GFP Lock MCR, BBa_K1379028: Cis-repressed GFP Generator HKUST Lock 1
- Lock 1 RBS GFP generator, BBa_K1379047: GFP generator using a Lock 3C modified RBS.
Data and submitted physical DNA for parts without qualified sample in the Part Registry:
- Inducible pBad/araC promoter, BBa_I0500: Inducible pBad/araC promoter.
- Riboregulator Lock 1, BBa_J01010:Biobrick version of Isaacs' riboregulator cis repressed lock, crR12.
- Lock3, BBa_J01080: Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1.
- Key3, BBa_J01086: Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1.
- Lock for medium RBS (B0032) from the lock/key library TUD09, BBa_K175031: This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.
- B0034 derived RBS to match spacing of J01122, BBa_J23080: RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.
- Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09, BBa_K175032: This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).
We also constructed the following parts:
- Riboregulator Key 1, BBa_J01008 (Golden Bear, 2005): BioBricked version of Isaacs' riboregulator trans-activating key, taR12
- Lock 3c, BBa_J23031 (Team Berkeley iGEM, 2006): lock3c.
- Key 3c, BBa_J23008 (Berkeley iGEM, 2006): Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP.
We constructed the following intermediates/measurement devices during characterization:
- Key 1 Double Terminator, BBa_K1379021
- Key 3 double terminator, BBa_K1379023
- Key 3c double terminator, BBa_K1379025
- MTA double terminator, BBa_K1379027
- HKUST Key 1 double terminator, BBa_K1379029
- Cis-Repressing device 1, BBa_K1379030
- AraC Key 1, BBa_K1379031
- Cis-Repressing device 3, BBa_K1379032
- AraC Key 3, BBa_K1379033
- Cis-Repressing device 3C, BBa_K1379051
- AraC Key 3C, BBa_K1379035
- Cis-Repressing device MCR, BBa_K1379036
- AraC MTA, BBa_K1379037
- Cis-Repressing device HKUST 1, BBa_K1379038
- AraC HKUST Key 1, BBa_K1379039
- Repression device 1, BBa_K1379040
- Repression device 3, BBa_K1379041
- Repression device 3C, BBa_K1379042
- Repression device Medium, BBa_K1379043
- Cis-Repression device HKUST 1, BBa_K1379044
- Cis repressed GFP lock 1, with Trans-activator key 1, BBa_K1379049
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