Team:Bielefeld-CeBiTec/Results/Outlook

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Module III - Isobutanol production

Isobutanol pathway

Alcohol dehydrogenase

As described in the section about the alcohol dehydrogenase we could show the successful overexpression of the protein AdhA from Lactococcus lactis in E. coli.
Further analysis could be an anaerobic cultivation for a complementation analysis of the strain ΔadhE748::kan. Trinh et al., 2011 showed that E. coli cannot grow after the loss of its alcohol dehydrogenase. Complemented with our plasmid (BBa_K1465305) the mutant should grow.

References
  • Trinh CT, Li J, Blanch HW, Clark DS., 2011. Redesigning Escherichia coli Metabolism for Anaerobic Production of Isobutanol. In: Appl Environ Microbiol., 77(14): 4894-904

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