Carboxysome

A carboxysome is a bacterial microcompartiment (BMC) surrounded by a protein shell. Naturally the occur in CO₂ fixing bacteria like cyanobacteria. Therefore they are the enzyme-containing organelles for these metabolic pathways (Bonacci et al., 2011; Shively et al., 1973). The first carboxysomes were discovered in 1956, and purified carboxysomes were first characterized in 1973 by Shively et al., 1973. Carboxysomes are formed by different shell proteins, surrounding an enzyme-containing lumen, which is rigorously separated from the cytoplasm. Thus, the possibility is given to enable reaction pathways incongruous with the conditions in the cytoplasm. The protein shell consists of two different types of proteins. Pentamers are used for the vertices of the icosaeder and hexamers for the facets. The main constituent of the shell is the CsoS1A protein, which contributes around 13 % of the total protein mass. Carboxysomes are between 80 and 120 nm in diameter (Kehrfeld et al., 2005). In the interior, there are two different types of enzymes. On the one hand there is the RuBisCO which catalyses the carboxylation or oxygenation of ribulose-1,5-bisphosphate. This reaction is one essential step in the Calvin cycle, the pathway for carbon fixation in autotrophic bacteria. On the other hand there is the carbonic anhydrase which converts hydrogen carbonate (HCO3^-) to carbon dioxide. The resulting carbon dioxide is the substrate for the RuBisCO. A schematic image of the carboxysome is given in Figure 1.

Generally, there are two different types of CBs found, called α-type (cso-carboxysome) and β-type (ccm-carboxysome) carboxysomes. This classification is based on differences in their component proteins and organization of their corresponding genes (Batger et al., 2002). α-type carboxysomes are found in α-cyanobacteria like some Synechococcus species or the chemoautotroph organism Halothiobacillus neapolitanus. This type of carboxysome contains the type I RuBisCO. Furthermore the respective genes for the carboxysome are arranged in a single operon. In β-type carboxysomes type II RuBisCO occurs, containing no small subunits. In contrast to α-type carboxysomes, the genes are arranged in multiple gene clusters. (Batger et al., 2002; Bonacci et al., 2011; Yeates et al., 2008) A possible difference in functionality between both types of carboxysomes is not fully understood (Yeates et al., 2008).

The advantage of the microcompartiment is the concentration of carbon dioxide in its lumen. Carboxyomes play a central role in carbon concentrating mechanisms (CCM) (Bonacci et al., 2011; Yeates et al., 2008) The reactions concerning the carboxysome are illustrated in figure 2. This mechanism is used for the accumulation of CO₂ in autotrophic prokaryotes. The carboxysome as a place of carbon dioxide fixation was first described in 1990 (Reinhold et al., 1990). The first reaction of the CCM is the transport of atmospheric carbon dioxide or hydrogencarbonate (HCO₃^-) into the cells. Accumulation of inorganic carbon is achieved by transmembrane pumps and transporters localisated in the cell membrane. At physiological pH values (approximately 7), the balance is clearly on the side of hydrogencarbonate. Hydrogencarbonate is able to diffuse across the shell of the carboxysome. The carbonic anhydrase (CA), located inside the carboxysome, catalyses the conversion of hydrogencarbonate to the substrate for RuBisCO, gaseous carbon dioxide. The CA is assosciated with the shell and presents only a small number of the carboxysome proteins. Thus, the CA, also named as CSOS3, acts to saturate the lumen with carbon dioxide and enables high effective concentrations of CO₂, so that the RuBisCO is able to work near its maximal reaction rate with high specifity. The formed product of the RuBisCO, 3-phosphoglycerate, is used in the metabolism of the cells. (Batger et al., 2002 (a); Bonacci et al., 2011; Yeates et al., 2008) The complete reaction is illustrated in figure 2. Diffusive loss of carbon dioxide is probably provided by the outer shell membrane acting as a barrier. How the shell is able to control the entry and exit of larger substrates is not fully understood (Yeates et al., 2008).

Figure 2: Reaction mechanism of the carboxysome. Hydrogencarbonate is converted to CO₂ catalyzed by the CA. CO₂ is fixated by the RuBisCO using ribulose-1,5-bisphosphate and so generating two molecules of 3-phosphoglycerate.

Halothiobacillus neapolitanus is a model organism for carboxysomes. This organism is a Gram-negative proteobacterium which belongs to the purple sulfur bacteria. It is obligate aerob and chemolitoautotroph. H. neapolitanus expresses the α-type carboxysome (cso-carboxysome). This type of carboxysome dominates in oligotrophic oceans. It has to be distinguished from the ccm-carboxysome (β-type). The first occurrence was 1957 by Parker et al. Recent studies have been made on this organism (Bonacci et al., 2011; Cannon, Shively 1983; Holthuijzen et al., 1986; So et al., 2004). The carboxysomal operon includes nine gene. One more gene, csoS1D was found, which is located outside of the core operon. (Bonacci et al., 2011) A structural overview of the nine genes including the tenth gene is shown in Figure 3.

Figure 3: Schematic mapping of the caboxysomal gene of Halothiobacillus neapolitanus. csoS1D is also shown here, although it is not found in the carboxysomal operon.

The genes for the RuBisCO are cbbL for the large subunit and cbbS for the small subunit. The corresponding proteins of RuBisCO compose nearly 70 % of the protein mass by weight (Yeates et al., 2008). The second gene coding for a protein with enzymatic activity is csoS3, the carbonic anhydrase.
The additional genes in the carboxysomal operon are related to the shell proteins. CsoS1A-C are the main shell proteins, building up the structure of the carboxysome. CsoS1A is responsible for the hexameric quaternary structure and is the major component of the CB shell. The gene csoS1D is not localized in the carboxysomal operon. It is suggested, that CsoS1D may modify function of CBs and influences the shell permeability. Furthermore it could be shown, that CsoS1D seems to reduce the number of CBs with shell defects. (Bonacci et al., 2011) CsoS2 is a another shell protein. With a size of 80-90 kDa it is a large protein, but the exact function is still unknown (Batger et al., 2002 (a); Yeates et al., 2008). CsoS2 is characteristic for α-type carboxysomes. It seems to have no enzymatic activity, but the localization primarily in the periphery of the carboxysome could be demonstrated (Yeates et al., 2008). CsoS4A and CsoS4B are responsible for the vertices of the icosahedral shell, which can be concluded from crystal structures. In the carboxysome both proteins are present equimolar, but in very low amounts. Knockout of both genes gives rise to more leaky carboxysomes, indicating a loss in the permeability barrier function of the shell. (Cai et al., 2009) In Table 1 a summary of the carboxysomal proteins also including CsoS1D is shown.

Carboxysomes enable efficient fixation of CO₂ in bacteria. For this reason, we want to use in our project the α-type carboxysome from the model organism Halothiobacillus neapolitanus and express it recombinant in E. coli. Therefore, we use the shown carboxysomal operon (figure 3) including csoS1D.

References

• Batger, Price 2002 (a). CO₂ concentrating mechanisms in cyanobacteria: molecular components, their diversity and evolution Journal of Experimental Botany, vol. 54, pp. 609-622
• Batger et al., 2002 (b). Evolution and diversity of CO₂ concentrating mechanisms in cyanobacteria Functional Plant Biology, vol. 29, pp. 161-173
• Bonacci, Walter, Poh K. Teng, Bruno Afonso, Henrike Niederholtmeyer, Patricia Grob, Pamela A. Silver, und David F. Savage. „Modularity of a Carbon-Fixing Protein Organelle“. Proceedings of the National Academy of Sciences 109, Nr. 2 (1. Oktober 2012): 478–83. doi:10.1073/pnas.1108557109.
• Cai et al., 2009. The Pentameric Vertex Proteins Are Necessary for the Icosahedral Carboxysome Shell to Function as a CO₂ Leakage Barrier PLoS ONE, vol.4, pp. 1-9
• Cannon, Shively 1983. Characterisation of a Homogenous Preparation of Carboxysomes from Thiobacillus neapolitanus. Archive of Microbiology, vol. 134, pp. 52-59
• Holthuijzen et al., 1986. Protein composition of the carboxysomes of Thiobacillus neapolitanus. Archive of Microbiology, vol. 144, pp. 398-404
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• Reinhold et al., 1990. A model for inorganic carbon fluxes and photosynthesis in cyanobacterial carboxysomes. Canadian Journal of Botany, vol. 69, pp. 984-988
• So et al., 2004. A Novel Evolutionary Lineage of Carbonic Anhydrase (ε Class) Is a Component of the Carboxysome Shell. Journal of Bacteriology, vol. 186, pp. 623-630
• Yeates et al., 2011. Protein-based organelles in bacteria: carboxysomes and related microcompartments. Nature Reviews Microbiology, vol. 6, pp. 681-691