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Team:UCL/Science/Primers
From 2014.igem.org
Primers for PpAzoR, PpAzoR1B6, BsCotA, BsDyP, PpDyP and BsAzoR for the purpose of adding the BioBrick prefix and suffix as shown in the figure below. The figures following represent the rationale behind each primer design
Our primer designs all aimed to optimise successful PCR. Because of try to design primers close to the following rules
- Primer melting temperature - Between 52-58oC.
- Primer annaeling temperature -
- GC Content - 40-60% G and C bases
-
The primers designed
The format of these primer designs is indicated right below.
FMN-dependent NADH-azoreductase
From Pseudomonas Putida (CDS = 612bp) Primer Design
From Pseudomonas Putida (CDS = 612bp) Two Site-directed Mutagenesis Designs
Heat-stable AzoR Mutant (AzoR 1B6)
From Pseudomonas Putida (CDS = 612bp) Primer Design
From Pseudomonas Putida (CDS = 612bp) Two site-directed Mutagenesis Designs
Spore coat protein laccase (cotA)
From Bacillus subtilis (CDS = 1539bp) Primer Design
From Bacillus subtilis (CDS = 1539bp) Two Site-Directed Mutagenesis Designs
Dye decolourising peroxidase BSU38260 (BsDyP)
From Bacillus subtilis (CDS = 1251bp) Primer Design
From Bacillus subtilis (CDS = 1251bp) Site-Directed Mutagenesis Designs
