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Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO
From 2014.igem.org
Module II - Carbon Dioxide (CO2) Fixation
Theory
The Ribulose 1,5-bisphosphate Carboxylase Oxygenase (RuBisCO)) is the most important enzyme in the Calvin cycle). It binds gaseous carbon dioxide to ribulose-1,5-bisphosphate (Ru-BP) generating two molecules of 3-phosphoglycerate (3-PGA). Therefore it is responsible for the fixation of carbon dioxide. 3-PGA is further converted in the Calvin cycle to glycerinaldehyde-3-phosphate. This is an essential intermediate in the central metabolism, as it plays a central role in glycolysis and gluconeogenesis. RuBisCO enzymes are chracterised as enzymes with slow reaction rates with a kcat of approximately 20. Furthermore they catalyse a side reaction with oxygen instead of of carbon dioxide, deteriorating the catalytic efficiency. The inclusion of the RuBisCO in a carboxysome, would significantly improve the efficiency of carbon fixation.
Figure1: Pathway of the D-xylose consumption in E. coli for hte fixation of carbon dioxide by the RuBisCO from Halothiobacillus neapolitnaus. For this approach the substrate ribulose 1,5-bisphosphate needs to be accumulated in the cell. This is realzied be the PrkA from Snyechoccous elongatus.
Thin Layer Chromatography
Cultivation
Bild Carbonat-Gleichgewicht
Bild Reaktor Schema
Bild Reaktor
Kalbriergerade
Kultivierung
