We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!

We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.

We sincerely thank for all the help given by School of Life Sciences and Biotechnology. During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.

Binhan Hao Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.

Ziyang Tan Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.