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Team:OUC-China/Project Result
From 2014.igem.org
mini plasmid & double plasmid system
mini plasmid
We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.
In the meantime, we ligate BBa_J04450 with BBa_J01003 to test the conjugated ability of BBa_J01003.
RP4 OriT 位点的失活
这一部分我们利用同源重组去失活RP4的OriT位点，很遗憾我们第一次的实验失败了，在比赛之前我们没有时间做第二次实验。在比赛结束后，我们会继续这部分的实验。但利用同源重组去失活OriT位点在理论上是完全行的通的。
