Week One

Tuesday, 6/17/14

• Get acquainted with summer logistics and lab basics
• Clean up lab space, stock lab supplies

Wednesday, 6/18/14

• Continue to discuss project and clean up lab space
• Designed PCR primers for combinatorial promoters subproject

Thursday, 6/19/14

• Formulated plasmid design (pAA001) for the response regulation subproject
• PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
• Gel electrophoresis for confirmation of PCR products' length

• With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14

• Purification of PCR products created yesterday
• Gibson assembly of purified products
• Transformation of Gibson-assembled plasmids into JM109 E. coli
• Meeting with Prof. Murray to discuss give an update on the project

• Colonies were found and picked after transformation and incubation.