OD/F Device
One of our greater visions we share with the synthetic biology community is that everyone interested in biological work should have access to basic laboratory equipment. With our OD/F Device we are able to offer a low cost solution for community labs, biohackers and high schools.
In general we have to make a difference between our OD and our F device. The reason for this is the bad comparability to commercial devices. These don't combine the measure of fluorescence and OD. With our OD/F we offer a solution to combine both measurement methods in one device. With our given instructions it is also possible to build two separate devices one for OD and one for F. In this context we offer the user a higher flexibility of choice. The user is also able to modify our device according to his own ideas and wishes.
Commercially obtainable spectrophotometers like [http://www.opticsplanet.com/unico-model-s-1205-spectrophotometer-5-nm-bandpass.html UNICO S-1205], for example, costs 1.249 $ or more, and can measure optical density only. At the same time they are heavy, not portable and therefore not easy to handle. But compared to our device it is able to work with a broader range of wavelengths.
Comparable to our developed device in the points measuring range and portability is the OD-meter [http://www.laboratory-equipment.com/laboratory-equipment/cell-density-meter.php CO 8000]. This device measures also the OD at 600 nm but still costs almost 920 $. Our OD/F Device can measure OD as well as fluorescence for less than 60 $.
Needed number of pieces, components and prices for creating your own OD or F device
OD/F device
|
| 1€=
| $1.27
| on 14/10/2014
|
|
Quantity | Component | Costs [€] | Costs [$] | Final [€] | Final [$]
|
1 | [http://www.dx.com/p/uno-r3-development-board-microcontroller-mega328p-atmega16u2-compat-for-arduino-blue-black-215600#.VDzwV9ysWBp Arduino UNO R3] | 9.17 | 11.65 | 9.17 | 11.65
|
1 | [http://www.mouser.com/ProductDetail/ams/TSL235R-LF/%3Fqs%3D14HO7rLZPQsjmBHaoYCzkA%253D%253D&sa=U&ei=3fA8VN3sN8T0OuLPgLAJ&ved=0CCUQ2yk&sig2=WOchotQO4XDym0jpXDjtzw&usg=AFQjCNGNr9DthURC_BKhgthh8EuJhjqutg TSL 235R] | 2.47 | 3.14 | 2.47 | 3.14
|
1 | [http://www.dx.com/p/16-x-2-character-lcd-display-module-with-blue-backlight-121356#.VDzxHNysWBp Display 16x2] | 2.58 | 3.28 | 2.58 | 3.28
|
1 | [http://www.dx.com/p/lcd1602-adapter-board-w-iic-i2c-interface-black-works-with-official-arduino-boards-216865#.VDzxHNysWBp LCD Display to I2C] | 1.57 | 1.99 | 1.57 | 1.99
|
1 | [http://www.newark.com/multicomp/mcpas6b1m1ce3/switch-pushbutton-spst-400ma-125v/dp/12P7696?ost=1638329 Pushbutton] | 2.90 | 3.69 | 2.90 | 3.69
|
1 | [http://shop.leefiltersusa.com/Swatch-Book-Designers-Edition-SWB.htm filter leaflet] | 1.57 | 2.00 | 1.57 | 2.00
|
20 | [http://www.dx.com/p/diy-male-to-female-dupont-breadboard-jumper-wires-black-multi-color-40-pcs-10cm-339078#.VDzxSdysWBp jumper wire cables] | 0.09 | 0.11 | 1.80 | 2.28
|
1 | [http://www.dx.com/p/syb-170-mini-breadboard-for-diy-project-red-140101#.VDzyudysWBo small breadboard] | 1.98 | 2.51 | 1.98 | 2.51
|
1 | [http://www.dx.com/p/universal-ac-charger-w-dual-usb-output-for-iphone-ipad-ipod-white-us-plug-244893#.VDzzMNysWBo power supply] | 2.20 | 2.80 | 2.20 | 2.80
|
1 | cuvette holder (3D print service of your choice) | 6.44 | 8.18 | 6.44 | 8.18
|
1 | 3 mm acrylic glas (black) | 7.98 | 10.14 | 7.98 | 10.14
|
1 | [http://www.dx.com/p/prototype-universal-printed-circuit-board-breadboard-golden-10-piece-pack-143913#.VDz3ttysWBo Prototype Universal Printed Circuit Board] | 2.27 | 2.88 | 2.27 | 2.88
|
1 | [http://www.dx.com/p/2-54mm-1x40-pin-breakaway-straight-male-header-10-piece-pack-144191#.VDz4HtysWBo Male Headers] | 2.14 | 2.72 | 2.14 | 2.72
|
| | | | |
|
1 | [http://www.mouser.de/ProductDetail/Dialight/550-2505F/?qs=0KZIkTEbAAvqMAW7suDOXg== LED 600 nm] | 0.99 | 1.11 | 0.87 | 1.11
|
Total OD | | | | 45.94 | 58.37
|
1 | [http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html LED 480 nm] | 0.99 | 1.26 | 0.99 | 1.26
|
Total F | | | | 46.06 | 58.52
|
1 | [http://www.mouser.de/ProductDetail/Dialight/550-2505F/?qs=0KZIkTEbAAvqMAW7suDOXg== LED 600nm] | 0.99 | 1.11 | 0.87 | 1.11
|
1 | [http://www.leds.de/Low-Mid-Power-LEDs/SuperFlux-LEDs/Nichia-Superflux-LED-blau-3lm-100-NSPBR70BSS.html LED 480 nm] | 0.99 | 1.26 | 0.99 | 1.26
|
Total OD/F | | | | 47.05 | 59.78
|
Cost saving lies here round about 850 $. All you need is to invest some time to construct our OD/F Device and this could also be a positive learning effect for schools and universities. Therefore, we present a solution for low budget institutions like schools, universities, community labs and the biohacking scene. Besides commercial [http://www.moleculardevices.com/systems/microplate-readers/fluorescent-readers/gemini-xps plate reader] are also available on the market, too. To compare our device with this comercial devices is really difficult. No portable devices like ours could be found which can also measure at a wave-band of 480 nm.
In our cooperation with the Kaiser-Karls-Gymnasium and with NEAnderLab one of our target groups gathered first experience using our OD/F device. As result we received the answer of our question if the device is useable in daily practice with teenagers. And we got a positive feedback for this. Even young people are able to use and understand our device.
|