Modeling
Prior to the experiments a model of our molecular approach was built to predict the results of our experiments.
The novel biosensor approach was modeled as shown above. The plotted results also include a model of a direct expression of GFP as it appears in traditional biosensors. The strength of the promotor used for the traditional approach is twice as high as the strength of the promotor upstream of the TEV coding sequence in our novel approach. Despite the stronger promotor, a higher GFP concentration is generated in the model of the novel biosensor, proving the stronger and faster fluorescence response of our construct in theory.
Since the final construct could not be built in time, a new model was designed according to the existing and functional double plasmid system. This is inducible with IPTG instead of 3-oxo-C12HSL as it contains the lac operon and is therefore a negative regulatory system.
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