DATA

Data For Our Favorite New Parts

Documented & submitted:

CpxR reporters were made with the promoter in forward and reverse direction, respectively BBa_K1486049 and BBa_K1486050.
A BioBrick consisting of CpxR under Arabinose Promoter was made, and then sfGFP was fused to CpxR's N terminus (BBa_K1486002) and C terminus (BBa_K1486005).
BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486002 )
BBa_K1486043 (LeuZ + rLuc http://parts.igem.org/Part:BBa_K1486043 )
BBa_K1486056 (Ga1Mut http:/parts.igem.org/Part:BBa_K1486056 )

Works as expected:

BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486008 )
BBa_K1486012 (CpxR IFP1 http://parts.igem.org/Part:BBa_K1486012 )
BBa_K1486013 (cpxR IFP2 http://parts.igem.org/Part:BBa_K1486013 )
BBa_K1486014 (IFP1 CpxR rLuc http://parts.igem.org/Part:BBa_K1486014 )
BBa_K1486015 (IFP2 CpxR rLuc http://parts.igem.org/Part:BBa_K1486015 )
BBa_K1486023 (Yeast optimized surperfolder GFP http://parts.igem.org/Part:BBa_K1486023 )
BBa_K1486024 (Yeast Kanamycin resistance http://parts.igem.org/Part:BBa_K1486024 )
BBa_K1486025 (ADH1 Terminator http://parts.igem.org/Part:BBa_K1486025 )
BBa_K1486026 (sfGFP + AHD1 terminator + Kanamycin resistance for yeast http://parts.igem.org/Part:BBa_K1486026 )
BBa_K1486027 (R.reniformis luciferase + ADH1 terminator + Kanamycin resistance http://parts.igem.org/Part:BBa_K1486027 )
BBa_K1486028 (Yeast optimized sfGFP N-terminus (1-214) http://parts.igem.org/Part:BBa_K1486028 )
BBa_K1486029 (sfGFPN + ADH1 terminator + Kanamycin resistance http://parts.igem.org/Part:BBa_K1486029)
BBa_K1486031 (CaUra3 selection marker http://parts.igem.org/Part:BBa_K1486031)
BBa_K1486034 (R.reniformis luciferase + ADH1 terminator + CaUra3 http://parts.igem.org/Part:BBa_K1486034 )
BBa_K1486035 (sfGFPC + ADH1 terminator + CaUra3 Cassette http://parts.igem.org/Part:BBa_K1486035 )

We realized that Calgary's CpxR reporter biobrick was missing the regulatory part of the sequence, so we repaired it and sent it as our BBa_K1486048. The BioBrick was further developed by testing the native CpxR target sequence that is found in front of CpxA in the E.coli genome (as Calgary's part did not include the whole sequence). These are BioBricks BBa_K1486049 and BBa_K1486050, with the promoter in forward and reverse direction respectively.
Submitted the two parts of the split of EPIC Firefly luciferase (N-terminal part (BBa_K1486016) and C-terminal part (BBa_K1486017)) from Cambridge 2010. The plasmid (BBa_K1486018) containing the two parts of the split separated by a spacer can be very useful as a negative control or to establish a background noise for a complementation assay experiment.
Compared the EPIC Firefly luciferase from Cambridge 2010 team to the renilla luciferase (BBa_K1486022) in the same conditions, to determine which one is best suited for a complementation assay experiment. The full and split luciferases has been compared. Renilla luciferase (full and splits(BBa_K1486021)) have been submitted.

Design of SmashColi - a testing chip to analyse the effects of different mechanical stresses on cells
Design of FilterColi - a testing chip to analyse the effects of different osmotic stresses on cells
Design of the BioPad - a large-scaled chip implemented to be the touch-senstive interface of our final trackpad
Design of CleanColi - blabla

Met with a journalist from the biggest newspaper of our region (Le Temps) and got an article about our project.
Our work was commented by Bent Stumpe, inventor of the touchscreen, as well as Rolf Heuer, the current director of the CERN, in Geneva.
Organized an outreach event with 80 highschool students at EPFL, teaching them about synthetic biology as well as laboratory techniques and made them participate in a game called « mini iGEM ».
Presented iGEM and our work at the Hackuarium, the new BioHackerspace in Lausanne.

Team:EPF Lausanne/Data