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Team:Bielefeld-CeBiTec/Results/Biosafety/Outlook
From 2014.igem.org
Biosafety
Remaining Challenges
So in conclusion it could be demonstrated, that the antibiotic-free selection system by via the D-alanine auxotrophic strain DH5α Δalr ΔdadX is not only possible, but even more efficent according to the transformation efficiency with the plasmid BBa_K1465401.
References
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Kang L, Shaw AC, Xu D, Xia W, Zhang J, Deng J, Wöldike HF, Liu Y, Su J. (2011) Upregulation of MetC is essential for D-alanine-independent growth of an alr/dadX-deficient Escherichia coli strain. Journal of bacteriology, vol. 193, pp. 1098 - 1106.
