Instruction manual GenTSV (S1) 

Instruction manual GefStoffV  

Legal regulations

Organisms

We have listed all used organisms in the strains&constructs section. Additional information about some bacteria are provided in the organisms section. More informaion about our constructed parts are located on the datapage. Our official spread sheet was submitted before the first of September.

Risks of Our Project

We are mainly working with safety strains of E.coli (K-12 derivatives) which are only toxic if injected into blood stream. It would also be problematic when these E. coli come in contact with the eyes. Oral exposition could change the bacterial population in the gut by delivering new DNA features. Lab safety training included safe handling regulations and mandatory lab coats and eye protection equipment during work with bacteria.
The definition of biosafety level 1 says that there is no risk according to the current scientific knowledge and if everything is done properly. The usage of safe E. coli strains should prevent any risks for the general public. DNA molecules from our strains could be transferred into other bacteria if genetically modified organisms escape the lab. The biggest problem are the antibiotic resistances, because they could be transferred to human pathogenic species. The impact of this problem is reduced by the fact that all resistance genes are already out there. Our lab safety trainings also included instructions on waste disposal requirements for normal and hazardous lab material as well as biologicals or lab material contaminated by biologicals. All genetically engineered strains and material contaminated with such organisms requires sterilisation by autoclaving. After this, the sterilised material is subjected to garbage incineration. Special decontamination rules regulate hazardous chemicals and material contaminated with such. The proper disposal of these is handled by the Department of Chemistry at our university. It would be possible to combine all our antibiotic resistances in one pathogenic organism to use it for bioterrorism purposes. We think, that the specific parts and constructs of our project and all possible combinations of these do not have an increased potential for malicious mis-use.
There are several levels of security measures. First of all, there is a personalized electronic access system for all labs, allowing a fine-grained restriction of access and a tracking of all access activities by the authorities of Bielefeld University.
In addition, the mandatory lab safety trainings and the continous surveillance of measures provides the second level of safety regulation. Next, there is the need for a detailed documentation of all experimental work, including all levels of accidents. Last, but not least, an atmosphere of responsible planning and conduct is pursued by all scientists, including the instructors and supervisors of the iGEM projects.

Risks of Our Project in the Future

The single parts of our project are, to our opinion, free of risks. This is implied by their biosafety level classification. We do not expect them to interact in a way which could increase these risks or provide unforseen ones. As stated above, we are planning to develop in-built safety systems, instantly killing the strain upon release from its containment. In addition, last year iGEM team has started to develop an antibiotics-free selection system and we worked on the improvement.
Additionally we are participating in SYNENERGENE. One important task during our participation is the development of possible senarios for the future of synthetic biology and the the development of application senarios. While doing this we contacted many experts and talk about possible biosafety issues assoziated with our project and future applications.