Team:Macquarie Australia/WetLab/Protocols/SDSPAGE

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Protocols


SDS-PAGE


  • Re-suspend pelleted bacterial cells in 200µL of Milli-Q-water
  • Transfer 50 µL of suspension into new Eppendorf tubes and combine with 50ul of 2xTruSep sample buffer.
  • Shear the cells using a Hamilton syringe.
  • Centrifuge the preparation for 3minutes @ 13,000 rpm.
  • Load 20 µL of the supernatant into gel.
  • Conduct electrophoresis at a constant voltage (200V) for 1 hour.
  • Coommassie Stain for ~30 minutes.

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