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Team:Macquarie Australia/WetLab/Protocols/SDSPAGE
From 2014.igem.org
SDS-PAGE
- Re-suspend pelleted bacterial cells in 200µL of Milli-Q-water
- Transfer 50 µL of suspension into new Eppendorf tubes and combine with 50ul of 2xTruSep sample buffer.
- Shear the cells using a Hamilton syringe.
- Centrifuge the preparation for 3minutes @ 13,000 rpm.
- Load 20 µL of the supernatant into gel.
- Conduct electrophoresis at a constant voltage (200V) for 1 hour.
- Coommassie Stain for ~30 minutes.
Figure 1. Protein bands from SDS-PAGE, visualized under UV light.
