Team:NRP-UEA-Norwich/Notebook Protocols

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NRP UEA Norwich iGEM 2014

Lab Protocols

GoldenGate Digestion-Ligation reaction (Level 1)

PROTOCOL HERE

GoldenGate Digestion-Ligation reaction (Level 2)

PROTOCOL HERE

Making LB Agar

PROTOCOL HERE

Making LB broth

PROTOCOL HERE

E. coli Calcium Chloride heat shock Transformation

PROTOCOL HERE

E. coli Electroporation Transformation

PROTOCOL HERE

Making elctrocompetent Agrobacterium tumefaciens

PROTOCOL HERE

Agrobacterium tumefaciens Electroporation Transformation

PROTOCOL HERE

Blue- White Selection

PROTOCOL HERE

Colony PCR

PROTOCOL HERE

DNA mini-prep

PROTOCOL HERE

Preparation for sequencing

PROTOCOL HERE

Agrobacterium tumefaciens Infiltration

PROTOCOL HERE

Infiltration Analysis

PROTOCOL HERE

Making GoldenGate compatible PSB1C3 'Flipper'

PROTOCOL HERE

Antibiotic Selection

PROTOCOL HERE
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