Team:UC Davis/Electrochemistry

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UC Davis iGEM 2014

Electrode Choice

Electrode Choice

System Optimization

System Optimization

Coupling Enzymes

Coupling Enzymes

The Electrochemistry team acquired, selected, and optimized an electrode setup for the detection of NADH at low concentrations in a complex solution. Additionally, the team demonstrated the ability of the electrode setup to detect enzyme generated NADH over time, and thereby functionally deconvolute aldehyde profiles within a sample.

Electrode Choice

Having settled on NAD+ dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH.
Our Electrode system needed to:

  • Detect NADH at low LOD (Sensitive)
  • Detect NADH with high linear range (Reactive)
  • Be robust to any possible solution components (Selective)
  • Be cheap (low cost)
  • Use a low sample volume (be efficient)
  • Be compatible and interfacaeble with potentiostat
  • Be Portable

System Optimization

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Coupling Enzymes

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