How our system works:
[Diagram with Biobrick Code]
Data For Our Favorite New Parts:
- σx, BBa_K1379004: gene sequence of competence protein ComX which produces σx factor. σx factor induces promoter containing Com-Box sequence (PcelA and PcomFA).
- PcelA promoter, BBa_K1379000: a σx-inducible promoter (containing Com-Box sequence) which initiates the transcription of competence CelA protein.
- PcomFA promoter, BBa_K1379001: a σx-inducible promoter (containing Com-Box sequence) which initiates the transcription of ComFA protein.
Data for Pre-existing Parts:
- Strong promoter, strong RBS combination, BBa_K880005 (Team Michigan iGEM, 2012): combination of strong constitutive promoter (BBa_J23100) and strong RBS (BBa_B0034) was used as a backbone for a very high level of expression of ComX protein (σx factor).
- GFP generator, BBa_E0240 (Antiquity Boston University iGEM, 2004): green fluorescent protein reporter (BBa_E0040) with RBS ( BBa_B0032) and double terminator ( BBa_B0015) was used as a reporter for characterization of PcelA and PcomFA promoters.
We Also Built and Characterized the Following Parts:
- σx Generator, BBa_K1379006: comX gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
- PcelA-GFP Generator, BBa_K1379002: σx-inducible PcelA promoter was assembled with GFP generator.
- PcomFA-GFP Generator, BBa_K1379003: σx-inducible PcomFA promoter was assembled with GFP generator.
- σx Generator, PcelA-GFP Generator, BBa_K1379005: σx-generator was assembled with PcelA-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
- σx Generator, PcomFA-GFP Generator, BBa_K1379007: σx-generator was assembled with PcomFA-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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