Team:The Tech Museum/Notebook

Notebook

Timeline of major events:
May: Basic concept research and design
June: Software development initiated; plasmid design started and promoter-RBS’s picked
July: Wetlab testing of possible color protein combinations
August 10: Design of complete tri-color plasmids finalized
August 24: Assembly of tri-color plasmid pools completed (DNA2.0)
September 10: Tri-color plasmids optimized on museum wetlab setup
September 29: Software fully integrated with hardware on mobile exhibit
September 30 - October 3: Prototyping of mobile exhibit with museum staff and visitors
October 6 - October 17: Data collection on the museum floor with visitor!

Biology Details

Design of tri-color plasmid pool (June 2014)
9 promoter-rbs pairs of varying strength from Kosuri et al paper (2013):