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Team:UESTC-China/Protocol
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Revision as of 04:13, 24 September 2014 by Zhangmanni (Talk | contribs)
DNA ligation with T4 DNA Ligase
| Component | 20ul Reaction |
|---|---|
| 1.33X Master Mix | 15ul |
| Insert DNA | the moles of insert DNA to vector DNA is 5：1 |
| >Vector DNA | 50ng |
| ddH2O | To 20ul |
| Incubate at 50℃ for 1 hour |
DNA ligation with T4 DNA Ligase
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
| XylS | BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO | Pseudomonas putida | xxxxxxxx | xxxxxxxxxxx |
