Team:Evry/Interlab Study/08-28-2014

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Picture

Contruction n°1: PSB1C3 with I20260




Contruction n°2: PSB1C3 with J23101-E1010




Contruction n°3: PSB1C3 with K823012-E1010


PCR colony was done on 7 colonies:
  1. Steril H2O:34,5µL
  2. 5X Q5 Reaction buffer: 10µL
  3. Template:2µL
  4. 10mM dNTP: 1µL
  5. 10µM Primer forward VF2: 1µL
  6. 10µM Primer reverse VR: 1µL
  7. Q5 DNA Polymerase: 0,5µL

A migration of PCR product was done. We expect to observe in each wells a band of 1176bp:
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6 colonies have a band at 1200 bp around so we decided to make the measurement with the third colony which was sent to sequence with the number of identification 26EA05 and 26EA06

Other constructions of the Anderson library of constitutive promoters


Promoters were resuspended from 2014 distribution kit plates with 10 µl of sterile MiliQ watter and stored at -20°C.
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Table: Additional promoters of the Interlab Study. In grey, plasmids already resuspended, transformed, sequenced and stock glycerol existed.

Aug 28

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