Team:Evry/Notebook/Transformation/09-01-2014

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Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation

pBBR1MCS pRhokHI-2 Ø plasmid
Pseudo replicate 1 Pseudo replicate 1 Pseudo
Pseudo replicate 2 Pseudo replicate 2 E.Coli Bl21
E.Coli Bl21 E.Coli Bl21

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection
Plates are showed and organised like shown in the following picture:
GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

A1 B1 C1 D1
Pseudo+pBBR1MCS (1) Pseudo+pBBR1MCS (2) Pseudo Pseudo Ø plasmid

A2 B2 C2 D2
Pseudo+pRhokHI-2 (1) Pseudo+pRhokHI-2 (2) Pseudo Pseudo Ø plasmid

A3 B3 C3
E.Coli Bl21 Bl21 Ø plasmid Bl21+pRhokHI-2

A4 B4 C4
E.Coli Bl21 Bl21 Ø plasmid Bl21+pBBR1MCS

We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture:
GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE

A B C D a b c d e f
E.Coli Bl21 Bl21 Ø plasmid Bl21+pBBR1MCS Bl21+pRhokHI-2 Pseudo Pseudo Ø plasmid Pseudo+pRhokHI-2 (1) Pseudo+pRhokHI-2 (2) Pseudo+pBBR1MCS (1) Pseudo+pBBR1MCS (2)

Aug 24

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