Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation
| pBBR1MCS |
pRhokHI-2 |
Ø plasmid |
| Pseudo replicate 1 |
Pseudo replicate 1 |
Pseudo |
| Pseudo replicate 2 |
Pseudo replicate 2 |
E.Coli Bl21 |
| E.Coli Bl21 |
E.Coli Bl21 |
|
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection
Plates are showed and organised like shown in the following picture:
| A1 |
B1 |
C1 |
D1 |
| Pseudo+pBBR1MCS (1) |
Pseudo+pBBR1MCS (2) |
Pseudo |
Pseudo Ø plasmid |
| A2 |
B2 |
C2 |
D2 |
| Pseudo+pRhokHI-2 (1) |
Pseudo+pRhokHI-2 (2) |
Pseudo |
Pseudo Ø plasmid |
| A3 |
B3 |
C3 |
| E.Coli Bl21 |
Bl21 Ø plasmid |
Bl21+pRhokHI-2 |
| A4 |
B4 |
C4 |
| E.Coli Bl21 |
Bl21 Ø plasmid |
Bl21+pBBR1MCS |
We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture:
| A |
B |
C |
D |
a |
b |
c |
d |
e |
f |
| E.Coli Bl21 |
Bl21 Ø plasmid |
Bl21+pBBR1MCS |
Bl21+pRhokHI-2 |
Pseudo |
Pseudo Ø plasmid |
Pseudo+pRhokHI-2 (1) |
Pseudo+pRhokHI-2 (2) |
Pseudo+pBBR1MCS (1) |
Pseudo+pBBR1MCS (2) |
Sep 01
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