Team:UT-Dallas/Notebook/8-14

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Revision as of 20:15, 20 August 2014

Thursday, August 14, 2014

Click here to edit the page

Today's tasks:
  • Miniprep ctxA, ctxB.
  • Test digest and ran gel for ctxA, ctxB.
  • Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
  • Prepared for sequencing: diluted to the correct volume and concentration.
  • Purified RBS (digested overnight yesterday).
  • Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
  • Inoculate more colonies from ctxA, ctxB plates.


RBS-Carb Gel was good.


Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.

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